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Shared by Tyler Wriston231 views

QProblem

In a lab manual I was provided with a table and told to calculate the values for the width of the FOV using the x10 and x40 objectives in both mm and micrometers. I watched a couple videos and still don't get it, i did try though, so my answer for the empty RELATIONSHIP column was 4, because I divided the x400 by x100 = 4. Then I filled in the first row for FOV by using this formula I found: FOVlow * Maglow = FOVhigh * Maghigh So I did 4 mm * 40 = FOVhigh * 100 160 = FOVhigh * 100 160/100 = 1.6 mm 1.6 * 1000 = 1600 micrometers Am i going right? HELP

ASolution

You’re very close. Let’s tidy the idea and then fill the table.

Key idea (simple rule):

  • Total magnification M=(ocular)×(objective)M = (\text{ocular}) \times (\text{objective}).
  • Field of view (FOV) scales inversely with magnification: FOV×M=constantFOV \times M = \text{constant}.
  • So if magnification goes up by a factor kk, FOV goes down by the same factor kk.

Your numbers:

  • With 10×10\times ocular:
    • Objective 4×4\times → total 40×40\times.
    • Objective 10×10\times → total 100×100\times.
    • Objective 40×40\times → total 400×400\times.

Relationship column (how many times bigger the higher magnification is compared to the first row at 40×40\times):

  • Row 1 (40×40\times vs 40×40\times): factor 11.
  • Row 2 (100×100\times vs 40×40\times): factor 100/40=2.5100/40 = 2.5.
  • Row 3 (400×400\times vs 40×40\times): factor 400/40=10400/40 = 10.

Now FOV values: We need one known FOV to anchor the rest. Your manual seems to give FOVFOV at 40×40\times as 4mm4\,\text{mm} (that’s what you used). If that’s the given, then:

  • Row 1 (40×40\times): FOV=4mm=4000μmFOV = 4\,\text{mm} = 4000\,\mu\text{m}.
  • Row 2 (100×100\times): divide by 2.52.5FOV=42.5=1.6mm=1600μmFOV = \frac{4}{2.5} = 1.6\,\text{mm} = 1600\,\mu\text{m}.
  • Row 3 (400×400\times): divide by 1010FOV=410=0.4mm=400μmFOV = \frac{4}{10} = 0.4\,\text{mm} = 400\,\mu\text{m}.

So yes, your calculation for the second row (1.6mm1.6\,\text{mm} and 1600μm1600\,\mu\text{m}) is right, given 4mm4\,\text{mm} at 40×40\times.

Quick check question: Does your lab manual explicitly give the FOVFOV at 40×40\times as 4mm4\,\text{mm}? If it gives a different starting FOV, we’ll scale all rows from that number using the same ratios.

What was the key insight here? That FOV×magnificationFOV \times \text{magnification} stays constant, so FOV changes by the inverse of the magnification factor.

Shared on 9/8/2025

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